Enzyme-catalyzed reaction path optimization and biosynthetic design based on genetic algorithm
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23 set 2025
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Pubblicato online: 23 set 2025
Ricevuto: 03 gen 2025
Accettato: 30 apr 2025
DOI: https://doi.org/10.2478/amns-2025-1110
Parole chiave
© 2025 Xiaoqian Zhang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1.
Figure 2.
Figure 3.
Figure 4.
The comparison of computed and experimental value of dynamic model
| Culture time(h) | Fungi dry weight(g/L) | CLA production(g/L) | Residual sugar(g/L) | |||
|---|---|---|---|---|---|---|
| Experimental value | Computed value | Experimental value | Computed value | Experimental value | Computed value | |
| 0 | 0.0067 | 0.0153 | 0.0000 | 0.0000 | 30.5400 | 30.5522 |
| 3 | 0.0712 | 0.0877 | 0.0000 | 0.0000 | 27.4436 | 27.4523 |
| 6 | 0.3390 | 0.3246 | 0.0000 | -0.0107 | 23.5625 | 23.5694 |
| 9 | 0.7547 | 0.7545 | 0.0019 | 0.0039 | 20.7066 | 20.7165 |
| 12 | 1.1476 | 1.1485 | 0.0647 | 0.0651 | 17.5478 | 17.5601 |
| 15 | 1.2526 | 1.2370 | 0.0609 | 0.0666 | 16.1969 | 16.2030 |
| 18 | 1.2840 | 1.2849 | 0.1548 | 0.1601 | 14.5413 | 14.5275 |
| 21 | 1.2854 | 1.2748 | 0.2907 | 0.3125 | 13.5045 | 13.5213 |
| 24 | 1.3314 | 1.3409 | 0.3496 | 0.3370 | 13.0553 | 13.0245 |
Orthogonal test of parameter optimization on producing CLA
| Factor | A | B | C | CLA production (g/L) |
|---|---|---|---|---|
| Number | LA concentration (%) | Ph value | Temperature | |
| 1 | 1.5(1) | 5(1) | 30(1) | 4.282±0.238 |
| 2 | 1.5(1) | 6(2) | 36(2) | 6.564±0.342 |
| 3 | 1.5(1) | 7(3) | 40(3) | 3.813±0.238 |
| 4 | 2(2) | 5(1) | 30(1) | 6.727±0.381 |
| 5 | 2(2) | 6(2) | 36(2) | 7.681±0.443 |
| 6 | 2(2) | 7(3) | 40(3) | 5.317±0.452 |
| 7 | 2.5(3) | 5(1) | 30(1) | 5.057±0.281 |
| 8 | 2.5(3) | 6(2) | 36(2) | 5.813±0.387 |
| 9 | 2.5(3) | 7(3) | 40(3) | 5.782±0.138 |
| R | 1.538 | 1.552 | 1.547 | |
| Superior level | ||||
| Factor sort | C>A>B | |||
| Best combination | ||||
Analysis of variance
| Factor | Deviation squares | df | Mean square sum | F | Sig. | Best combination |
|---|---|---|---|---|---|---|
| A | 3.64 | 2 | 1.82 | 72.8 | <0.05 | |
| B | 3.46 | 2 | 1.73 | 70.2 | <0.05 | |
| C | 4.76 | 2 | 2.38 | 87.9 | <0.05 | |
| Test error | 0.05 | 2 | 0.025 | |||
| Sum. | 11.91 | 8 |