Enzyme-catalyzed reaction path optimization and biosynthetic design based on genetic algorithm
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Sep 23, 2025
About this article
Published Online: Sep 23, 2025
Received: Jan 03, 2025
Accepted: Apr 30, 2025
DOI: https://doi.org/10.2478/amns-2025-1110
Keywords
© 2025 Xiaoqian Zhang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1.
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Figure 3.
Figure 4.
The comparison of computed and experimental value of dynamic model
| Culture time(h) | Fungi dry weight(g/L) | CLA production(g/L) | Residual sugar(g/L) | |||
|---|---|---|---|---|---|---|
| Experimental value | Computed value | Experimental value | Computed value | Experimental value | Computed value | |
| 0 | 0.0067 | 0.0153 | 0.0000 | 0.0000 | 30.5400 | 30.5522 |
| 3 | 0.0712 | 0.0877 | 0.0000 | 0.0000 | 27.4436 | 27.4523 |
| 6 | 0.3390 | 0.3246 | 0.0000 | -0.0107 | 23.5625 | 23.5694 |
| 9 | 0.7547 | 0.7545 | 0.0019 | 0.0039 | 20.7066 | 20.7165 |
| 12 | 1.1476 | 1.1485 | 0.0647 | 0.0651 | 17.5478 | 17.5601 |
| 15 | 1.2526 | 1.2370 | 0.0609 | 0.0666 | 16.1969 | 16.2030 |
| 18 | 1.2840 | 1.2849 | 0.1548 | 0.1601 | 14.5413 | 14.5275 |
| 21 | 1.2854 | 1.2748 | 0.2907 | 0.3125 | 13.5045 | 13.5213 |
| 24 | 1.3314 | 1.3409 | 0.3496 | 0.3370 | 13.0553 | 13.0245 |
Orthogonal test of parameter optimization on producing CLA
| Factor | A | B | C | CLA production (g/L) |
|---|---|---|---|---|
| Number | LA concentration (%) | Ph value | Temperature | |
| 1 | 1.5(1) | 5(1) | 30(1) | 4.282±0.238 |
| 2 | 1.5(1) | 6(2) | 36(2) | 6.564±0.342 |
| 3 | 1.5(1) | 7(3) | 40(3) | 3.813±0.238 |
| 4 | 2(2) | 5(1) | 30(1) | 6.727±0.381 |
| 5 | 2(2) | 6(2) | 36(2) | 7.681±0.443 |
| 6 | 2(2) | 7(3) | 40(3) | 5.317±0.452 |
| 7 | 2.5(3) | 5(1) | 30(1) | 5.057±0.281 |
| 8 | 2.5(3) | 6(2) | 36(2) | 5.813±0.387 |
| 9 | 2.5(3) | 7(3) | 40(3) | 5.782±0.138 |
| R | 1.538 | 1.552 | 1.547 | |
| Superior level | ||||
| Factor sort | C>A>B | |||
| Best combination | ||||
Analysis of variance
| Factor | Deviation squares | df | Mean square sum | F | Sig. | Best combination |
|---|---|---|---|---|---|---|
| A | 3.64 | 2 | 1.82 | 72.8 | <0.05 | |
| B | 3.46 | 2 | 1.73 | 70.2 | <0.05 | |
| C | 4.76 | 2 | 2.38 | 87.9 | <0.05 | |
| Test error | 0.05 | 2 | 0.025 | |||
| Sum. | 11.91 | 8 |